mouse monoclonal anti proliferating cell nuclear antigen pcna  (Proteintech)

Journal: Oxidative Medicine and Cellular Longevity

Article Title: Apoptosis in the Mammary Gland of Virgin Rats Subchronically Fed With a Vitamin A Deficient Diet

doi: 10.1155/omcl/6334165

Figure Lengend Snippet: VAD and further refeeding with a VAS diet cause changes in cell proliferation in the mammary gland. (A) Immunohistochemistry for PCNA in the mammary gland. The black arrows indicate positive PCNA + cells. Magnification 100×. The scale bar represents 25 μm. (B) Quantification of the percentage of PCNA + cells from the immunohistochemistry images shown in (A). Data are shown as representative images or mean values ± SDs ( n = 10).

Article Snippet: The sections were incubated with the following primary antibodies: 8 h in a humidified chamber at 4°C with mouse monoclonal antihuman BCL-2 (clon BCL-2/100; Catalog. No. AM287-5M, BioGenex, San Ramón, CA, USA), 30 min in a humidified chamber at 20°C with rabbit polyclonal antihuman BAX protein (Catalog. No. AR347-5R, BioGenex, San Ramón, CA, USA), and for 4 h in a humidified chamber at 20°C with mouse monoclonal anti-rat proliferating cell nuclear antigen (PCNA) (clon PC10; Catalog. No. AM252-5M, BioGenex, San Ramón, CA, USA).

Techniques: Immunohistochemistry

Journal: Oxidative Medicine and Cellular Longevity

Article Title: Apoptosis in the Mammary Gland of Virgin Rats Subchronically Fed With a Vitamin A Deficient Diet

doi: 10.1155/omcl/6334165

Figure Lengend Snippet: VAS and further refeeding with a VAS diet cause changes in apoptosis and proliferation in the mammary gland. Determination of the TUNEL + /PCNA + cell ratios from the immunohistochemistry images shown in Figures A and A. The data are presented as the means ± SDs ( n = 4).

Article Snippet: The sections were incubated with the following primary antibodies: 8 h in a humidified chamber at 4°C with mouse monoclonal antihuman BCL-2 (clon BCL-2/100; Catalog. No. AM287-5M, BioGenex, San Ramón, CA, USA), 30 min in a humidified chamber at 20°C with rabbit polyclonal antihuman BAX protein (Catalog. No. AR347-5R, BioGenex, San Ramón, CA, USA), and for 4 h in a humidified chamber at 20°C with mouse monoclonal anti-rat proliferating cell nuclear antigen (PCNA) (clon PC10; Catalog. No. AM252-5M, BioGenex, San Ramón, CA, USA).

Techniques: TUNEL Assay, Immunohistochemistry

Journal: International Journal of Molecular Sciences

Article Title: The Vasopressin Receptor Antagonist Tolvaptan Counteracts Tumor Growth in a Murine Xenograft Model of Small Cell Lung Cancer

doi: 10.3390/ijms25158402

Figure Lengend Snippet: Western blot and IHC analysis for PCNA and caspase-3. ( a ) Western blot analysis of PCNA protein expression in tumor masses of control and tolvaptan mice: images represent blots, whereas scatter dot plots on right show normalized PCNA vs. stain-free gel (* = p ≤ 0.05 vs. control group). ( b ) PCNA IHC on tissue sections obtained from tumors: representative images are shown on left, whereas scatter plot on right illustrates the densitometric analysis of PCNA-positive cells (** = p ≤ 0.02 vs. control group). ( c ) Western blot analysis of caspase-3 in tumor masses of control and tolvaptan mice: images on left represent blots, whereas scatter dot plots on right show the densitometric analysis of normalized protein expression vs. stain-free gel (* = p ≤ 0.05 vs. control group). ( d ) Caspase-3 IHC of tissue sections obtained from tumors: representative images are shown on left, whereas scatter dot blots on right illustrate densitometric analysis of caspase-3-positive cells (* = p ≤ 0.05 vs. control group).

Article Snippet: The primary antibodies used were mouse monoclonal anti-Proliferating Cell Nuclear Antigen (PCNA) (#2586S, 1:100, Cell Signaling Technology, Danvers, MA, USA), rabbit polyclonal anti-caspase-3 (#9661, 1:100, Cell Signaling Technology, Danvers, MA, USA), and rat monoclonal anti-CD34 (ab8158, 1:50, Abcam, Cambridge, UK).

Techniques: Western Blot, Expressing, Control, Staining

Journal: Plastic & Reconstructive Surgery

Article Title: Tracing the Change and Contribution of Subcutaneous Adipose to Skin Expansion Using a Luciferase-Transgenic Fat Transplantation Model

doi: 10.1097/prs.0000000000010753

Figure Lengend Snippet: Fig. 4. Subcutaneous adipose tissue promoted vascularization and cell proliferation in expanded skin by means of paracrine growth factors. (Above) Immunohistochemical staining for CD31 and PCNA to mark ves- sels and proliferating cells. (Center) mRNA expression levels of EGF, bFGF, and VEGF in skin samples from the control group and the AT group (AT-Skin), and adipose tissue from the AT group (AT-Adipose). (Below, right) Immunoblot analysis of the expression of bFGF, EGF, and VEGF in skin samples from the control group and the AT group, and adipose tissue from the AT group. Scale bar = 100 µm; *P < 0.05; ***P < 0.001.

Article Snippet: To detect the vessels and proliferating cells (early G1 and S phases) in expanded skin, rabbit anti-rat CD31 (BM2966; Boster, Wuhan, People’s Republic of China) and mouse anti-rat proliferating cell nuclear antigen (PCNA) (BM0104; Boster) antibodies were applied.

Techniques: Immunohistochemical staining, Staining, Expressing, Control, Western Blot